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surface stem cell marker tra1 60  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec surface stem cell marker tra1 60
    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
    Surface Stem Cell Marker Tra1 60, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+surface+antibodies/TRA-1-60+Antibody%2C+anti-human%2C+REAfinity/pmc13352860-130-13-20
    Average 95 stars, based on 42 article reviews
    surface stem cell marker tra1 60 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing"

    Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-026-05174-3

    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
    Figure Legend Snippet: Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

    Techniques Used: Virus, Derivative Assay, Staining, Generated, Flow Cytometry, Control, Expressing

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    Staining:

    Article Title: A landscape of patient-derived cancer-associated fibroblast signals in endometrial cancers
    Article Snippet: .. Cells were stained for 15 minutes with cell surface antibodies (CD31 Miltenyi, CD155 Miltenyi, CD90 Miltenyi, FAP R&D systems, PD-L1 Miltyeni) or corresponding isotype control antibodies (Miltenyi). .. Cells were fixed using the kit from Miltenyi for 30 minutes, followed by re-suspension in a permeabilization buffer from the same kit.

    Article Title: A CAF-Based Two-Cell Hybrid Co-Culture Model to Test Drug Resistance in Endometrial Cancers
    Article Snippet: .. Cells were stained for 15 min with cell surface antibodies or corresponding isotype control antibodies (Miltenyi). ..

    Article Title: Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs.
    Article Snippet: .. Cells were stained for 15 min with cell surface antibodies (CD31 Miltenyi, CD155 Miltenyi, CD90 Miltenyi, FAP R&D systems, PD-L1 Miltyeni) or corresponding isotype control antibodies (Miltenyi). .. Cells were fixed using the kit from Miltenyi for 30 min, followed by re-suspension in a permeabilization buffer from the same kit.

    Article Title: Mucosal-Associated Invariant T Cells Display Diminished Effector Capacity in Oesophageal Adenocarcinoma
    Article Snippet: Cells were harvested, washed in PBA solution and accumulated intracellular cytokines were measured using a Fixation/Permeabilisation Solution kit (BD Biosciences), as per manufacturer's recommendations. .. In brief, cells were stained with cell surface antibodies (PD-1 PE, CD8 PerCP, Vα7.2 PEVio770, CD3 VioGreen, CD161 APC), (Miltenyi Biotec), were washed, permeabilised, and then stained for intracellular cytokines (IFN-γ FITC, IL-17A VioBlue, and TNF-α APCCy7), (Miltenyi Biotec). .. Cells were acquired on a FACS Canto flow cytometer (BD Biosciences).

    Article Title: Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs
    Article Snippet: .. Cells were stained for 15 min with cell surface antibodies (CD31 Miltenyi, CD155 Miltenyi, CD90 Miltenyi, FAP R&D systems, PD-L1 Miltyeni) or corresponding isotype control antibodies (Miltenyi). .. Cells were fixed using the kit from Miltenyi for 30 min, followed by re-suspension in a permeabilization buffer from the same kit.

    Control:

    Article Title: A landscape of patient-derived cancer-associated fibroblast signals in endometrial cancers
    Article Snippet: .. Cells were stained for 15 minutes with cell surface antibodies (CD31 Miltenyi, CD155 Miltenyi, CD90 Miltenyi, FAP R&D systems, PD-L1 Miltyeni) or corresponding isotype control antibodies (Miltenyi). .. Cells were fixed using the kit from Miltenyi for 30 minutes, followed by re-suspension in a permeabilization buffer from the same kit.

    Article Title: A CAF-Based Two-Cell Hybrid Co-Culture Model to Test Drug Resistance in Endometrial Cancers
    Article Snippet: .. Cells were stained for 15 min with cell surface antibodies or corresponding isotype control antibodies (Miltenyi). ..

    Article Title: Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs.
    Article Snippet: .. Cells were stained for 15 min with cell surface antibodies (CD31 Miltenyi, CD155 Miltenyi, CD90 Miltenyi, FAP R&D systems, PD-L1 Miltyeni) or corresponding isotype control antibodies (Miltenyi). .. Cells were fixed using the kit from Miltenyi for 30 min, followed by re-suspension in a permeabilization buffer from the same kit.

    Article Title: Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs
    Article Snippet: .. Cells were stained for 15 min with cell surface antibodies (CD31 Miltenyi, CD155 Miltenyi, CD90 Miltenyi, FAP R&D systems, PD-L1 Miltyeni) or corresponding isotype control antibodies (Miltenyi). .. Cells were fixed using the kit from Miltenyi for 30 min, followed by re-suspension in a permeabilization buffer from the same kit.

    FACS:

    Article Title: Patient-Derived Primary Cancer-Associated Fibroblasts Mediate Resistance to Anti-Angiogenic Drug in Ovarian Cancers.
    Article Snippet: .. Cell number was adjusted to 106 cells per sample, resuspended in FACS buffer along with corresponding cell surface antibodies (FAP-PE R&D systems, FAB3715P; and FAP-AF488 R&D systems FAB3715G, PD-L1: 130-122-810 from Miltenyi), and incubated at 4 ◦C for 20 min (FAP-FITC; SMA-PE, Novus NBP2-34760PE; S100A4-PerCP: NBP2-54580PCP; PD-L1-APC: 130-122-810). .. Cells were rinsed twice with FACS buffer, then fixed for 30 min, rinsed and resuspended in permeabilization buffer (Fix/Perm kit Miltenyi), and the corresponding intracellular antibodies were added for 30 min (SMA: NBP2-34760F and S100A4: NBP254580PCP, Novus).

    Article Title: Patient-Derived Primary Cancer-Associated Fibroblasts Mediate Resistance to Anti-Angiogenic Drug in Ovarian Cancers
    Article Snippet: .. Cell number was adjusted to 10 6 cells per sample, resuspended in FACS buffer along with corresponding cell surface antibodies (FAP-PE R&D systems, FAB3715P; and FAP-AF488 R&D systems FAB3715G, PD-L1: 130-122-810 from Miltenyi), and incubated at 4 °C for 20 min (FAP-FITC; SMA-PE, Novus NBP2-34760PE; S100A4-PerCP: NBP2-54580PCP; PD-L1-APC: 130-122-810). .. Cells were rinsed twice with FACS buffer, then fixed for 30 min, rinsed and resuspended in permeabilization buffer (Fix/Perm kit Miltenyi), and the corresponding intracellular antibodies were added for 30 min (SMA: NBP2-34760F and S100A4: NBP2-54580PCP, Novus).

    Incubation:

    Article Title: Patient-Derived Primary Cancer-Associated Fibroblasts Mediate Resistance to Anti-Angiogenic Drug in Ovarian Cancers.
    Article Snippet: .. Cell number was adjusted to 106 cells per sample, resuspended in FACS buffer along with corresponding cell surface antibodies (FAP-PE R&D systems, FAB3715P; and FAP-AF488 R&D systems FAB3715G, PD-L1: 130-122-810 from Miltenyi), and incubated at 4 ◦C for 20 min (FAP-FITC; SMA-PE, Novus NBP2-34760PE; S100A4-PerCP: NBP2-54580PCP; PD-L1-APC: 130-122-810). .. Cells were rinsed twice with FACS buffer, then fixed for 30 min, rinsed and resuspended in permeabilization buffer (Fix/Perm kit Miltenyi), and the corresponding intracellular antibodies were added for 30 min (SMA: NBP2-34760F and S100A4: NBP254580PCP, Novus).

    Article Title: Patient-Derived Primary Cancer-Associated Fibroblasts Mediate Resistance to Anti-Angiogenic Drug in Ovarian Cancers
    Article Snippet: .. Cell number was adjusted to 10 6 cells per sample, resuspended in FACS buffer along with corresponding cell surface antibodies (FAP-PE R&D systems, FAB3715P; and FAP-AF488 R&D systems FAB3715G, PD-L1: 130-122-810 from Miltenyi), and incubated at 4 °C for 20 min (FAP-FITC; SMA-PE, Novus NBP2-34760PE; S100A4-PerCP: NBP2-54580PCP; PD-L1-APC: 130-122-810). .. Cells were rinsed twice with FACS buffer, then fixed for 30 min, rinsed and resuspended in permeabilization buffer (Fix/Perm kit Miltenyi), and the corresponding intracellular antibodies were added for 30 min (SMA: NBP2-34760F and S100A4: NBP2-54580PCP, Novus).



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    Developmental Studies Hybridoma Bank anti drp1
    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
    Anti Drp1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Miltenyi Biotec astrocyte cell surface antigen 1 acsa 1 biotin
    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% <t>SSEA4/TRA1-60,</t> G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)
    Astrocyte Cell Surface Antigen 1 Acsa 1 Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+surface+antibodies/Anti-GLAST+(ACSA-1)+MicroBead+Kit%2C+human%2C+mouse/pm41645892-113-44-49
    Average 95 stars, based on 1 article reviews
    astrocyte cell surface antigen 1 acsa 1 biotin - by Bioz Stars, 2026-09
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    Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

    Journal: Stem Cell Research & Therapy

    Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing

    doi: 10.1186/s13287-026-05174-3

    Figure Lengend Snippet: Reprogramming of adult human dermal fibroblasts (HDFa) into induced pluripotent stem cell (iPSC) line BO-VC1. Reprogramming was performed using the Epi5 ™ Episomal iPSC Reprogramming Kit, enabling the generation of ( A ) transgene- and virus-free iPSC line BO-VC1. Successful generation of fibroblast-derived iPSCs was validated by immunocytochemical staining for the pluripotency markers ( B ) SOX2, C TRA 1–60, D OCT4, SSEA4 and E NANOG. Quantification of the generated iPSCs via flow cytometry revealed F 99.31% SSEA4/TRA1-60, G 97.43% SOX2/TRA1-60 and H 98.60% OCT3/4/TRA1-60 positive cells. I Quantification of the transcript levels of the stem cell markers NANOG , OCT4 , REX1 and SOX2 revealed significantly higher mRNA levels in iPSC line BO-VC1 compared to the HDFa control ( n = 3 different passages) Moreover, generated iPSCs were functionally validated by directed differentiation into all three germ layers. Subsequent immunocytochemical staining of the iPSCs before and after differentiation confirmed the expression of ( J–M ) meso-, N–Q endo- and R–U ectodermal lineage markers only in the respective differentiations. Scale bars: 50 μm. Data were tested for normal distribution using Shapiro-Wilk test. Means ± SEM (standard error of the mean) were statistically analyzed by a Kruskal-Wallis test with Dunn’s multiple comparisons test. n = 3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001)

    Article Snippet: After harvesting, using Accutase, 1 × 10 6 cells were stained for the surface stem cell marker TRA1-60 (1:50, #130-122-965, Miltenyi Biotec, Bergisch Gladbach, Germany) and SSEA4 (1:50, #130-124-073, Miltenyi Biotec, Bergisch Gladbach, Germany) in 25 μl PEB buffer (PBS + 0.5% BSA) for 10 min at 4 °C, washed in 500 μl PEB buffer and centrifuged at 200xg for 5 min.

    Techniques: Virus, Derivative Assay, Staining, Generated, Flow Cytometry, Control, Expressing